TITLE:
Identification Cold Tolerance of Pineapple Germplasms at Seedling Stage
AUTHORS:
Haiyan Shu, Keming Li, Yanfei Ou, Rulin Zhan, Shenghe Chang
KEYWORDS:
Pineapple, Cold Tolerance, Seedling Stage, Simple Sequence Repeat (SSR), Breeding
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.12 No.12,
December
8,
2021
ABSTRACT: Pineapple plant was always injured due to low temperature.
Breeding varieties with high cold tolerance was the supreme way to resolve this
problem and identifying cold tolerance of germplasms collected was necessary.
In this study, SSR locus around homologous sequences of cold-tolerant genes was searched and screened using
MD-2 (cold-tolerant variety) and Tainong 17 (cold-sensitive variety). Seventy
three pairs of primers of which PCR results were different between these two varieties
were gotten. PCRs were performed using these as primers and genomic DNA of
germplasms collected as templates. Six pairs of primers were found that their
PCR results were in good consistent with cold tolerance. Using GLM (General
linear model) association mapping analysis,
two SSR markers linked to cold tolerance were found. Their efficiency was
verified further using pineapple germplasms with high cold tolerance which had
been validated in field by cold wave. They will be used in cold-tolerant breeding
of pineapple in the future.