TITLE:
Identification and Characterization of Simple Sequence Repeats (SSR) Markers Associated with Downy Mildew Resistance Locus “RPV1” in Grapes
AUTHORS:
Neetu Goyal, Akshay Nag, Roshni R. Samarth, Anuradha Upadhyay, Kashmir Singh
KEYWORDS:
Downy Mildew, Resistance, Locus, Simple Sequence Repeats, Genotyping
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.12 No.11,
November
29,
2021
ABSTRACT: The cultivation of grapes is severely impacted by the
emergence of downy mildew (DM) disease which negatively affects quality and
yield possibly resulting in heavy losses. Due to certain shortcomings in the
usage of fungicides and the development of new cultivars by plant breeding,
marker assisted selection (MAS) will be an efficient alternative method to
introduce desired genes into the cultivated varieties in a short time period.
The Simple sequence repeats (SSR) markers seem to be the most popular genetic
marker of choice for MAS. In the present study, we identified 14 new SSR
markers in RPV1 locus that are associated with downy mildew resistance
in grapes. The characterization of the identified markers was carried out on
the basis of various parameters such as types of repeat motifs, number of
repeats, different classes and structure of microsatellites. Additionally, SSR
genotyping in 56 different grape accessions was done to determine the
susceptibility or resistance of these accessions to DM.