TITLE:
Influence of Watering Solution and Phenotype on the Growth of in Vitro Propagated Pineapple (Smooth Cayenne Cultivar) Plantlets during Acclimatization
AUTHORS:
Arnaud Agbidinoukoun, Gaston C. Ouikoun, Toussaint Mikpon, Gatien T. Kamade, Bienvenu T. Badou, Richmy C. B. Aïsso, Serge S. Houedjissin, Jerome A. Houngue, René C. Dossoukpevi, Corneille Ahanhanzo
KEYWORDS:
Ananas comosus var. comosus, Plantlets Morphology, Nutrient Supply, Pineapple Acclimatization, In Vitro Tissue Culture
JOURNAL NAME:
Agricultural Sciences,
Vol.12 No.11,
November
10,
2021
ABSTRACT: Pineapple is the first fruit crop cultivated in south Benin that greatly contributes for food and nutritional security and farmers’ income. But the lack of homogenous planting material constitutes the major constraint for improving pineapple yield. In vitro micropropagation is now used in the production of homogenous and free disease planting materials of pineapple. However, the acclimatization to natural condition of pineapple plantlets is an important step in planting material production of this crop. Here, we determined the intrinsic and extrinsic factors which influence the behavior of plantlets during the acclimatization process. For this purpose, plantlets from different categories were selected, trimmed and planted on a horticultural substrate made up of potting soil, white sawdust and compost previously sterilized. The plantlets were then incubated in under acclimatization greenhouse with average temperature of 29˚C and 70.2% of humidity. A batch of plantlets was subjected to two different watering solutions: Shive and Robbins solution and NPK 14-6-5 foliar fertilizer. The results obtained initially showed high rate (100%) of survival and growth of the plantlets watered with Shive and Robbins solution against 50% of the plantlets watered with the foliar fertilizer solution. In addition, the plantlets with spread pores exhibiting the characteristics of which the number of leaves varies between 9 and 11, the weight between 1.2 and 1.5 g, the size of 4.5 to 5.5 cm, and a good junction between the aerial part and the root system were those which were successfully grown in acclimatization phase under greenhouse, unlike plantlets with erected pores having lower success rate. This study goes a long way in providing good procedures of acclimatization of homogenous and free disease planting material of pineapple to the famers.