TITLE:
Dermaseptin B2’s Anti-Proliferative Activity and down Regulation of Anti-Proliferative, Angiogenic and Metastatic Genes in Rhabdomyosarcoma RD Cells in Vitro
AUTHORS:
Ahmed A. Abdille, Josephine Kimani, Fred Wamunyokoli, Wallace Bulimo, Yahaya Gavamukulya, Esther N. Maina
KEYWORDS:
Dermaseptin B2, Doxorubicin, RMS, Angiogenesis, Metastasis
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.12 No.10,
October
28,
2021
ABSTRACT: Background: Rhabdomyosarcoma (RMS) is the most prevalent soft tissue sarcoma in
children, representing approximately 50% of pediatric sarcomas and can develop
in any part of the body though more frequently at the extremities. Aim: Evaluating
the in vitro anti-proliferative
activity of Dermaseptin B2 on Rhabdomyosarcoma RD (CCL-136TM) cells and its
effect on the expression of MYC, FGFR1,
NOTCH1, and CXCR7 genes involve in processes including
proliferation, angiogenesis and metastasis. Methods: RD cells were grown
in Dulbecco’s Modified Eagle’s Medium supplemented with 10% Fetal Bovine Serum.
Exponentially growing cells were treated with Dermaseptin B2 and
Antiproliferative activity was assayed using the resazurin and migration assays
at three time-points. In order to determine the gene expression profiles of MYC,
NOTCH1, FGFR1 and CXCR7, total RNA was extracted from the cells and
q-RT-PCR was performed with β-Actin as reference gene. Results: Dermaseptin
B2 inhibited the proliferation of RD cells in a time and concentration
dependent manner as with IC50 values of 7.679 μM, 7.235 μM, 5.993 μM. The 2-dimentional wound
healing assay showed inhibition of migration and motility of the RD cells at
time-points of 6, 24, 48 and 72-hours with the greatest inhibition observed at
72-hours. Dermaseptin B2 downregulated the target MYC (fc; 1.5013,
1.5185, 2.4144), CXCR7 (fc; 2.8818, 4.4430, 3.9924), FGFR1 (fc;
2.3515, 2.0809, 2.2543), NOTCH1 (fc; 2.4667, 4.6274, 4.3352) genes for
the three-time points respectively. NOTCH1 and CXCR7 showed
higher fold changes with respect to β-Actin than MYC and FGFR1. Conclusion: The results of this study indicate that Dermaseptin B2 is a target molecule for signaling pathways including
PI3K/AKT, RTK and NOTCH pathways that could affect the transcription of these
genes and overall inhibition of cancer progression. Further studies are needed
to give a better understanding of the detailed mechanisms of action as well as
the effects of the Dermaseptin B2 peptide in vivo.