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Bruijins, B., Tiggelaar, R. and Gardeniers, H. (2017) Dataset of the Absorption, Emission and Excitation Spectra and Fluorescence Intensity Graphs of Fluorescent Cyanine Dyes for the Quantification of Low Amounts of dsDNA. Data in Brief, 10, 132-143. https://doi.org/10.1016/j.dib.2016.11.090

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• TITLE: A Novel Label-Free Fluorescence Strategy Based on Dumbbell Probe for Sensitive Detection of DNA Ligase

  AUTHORS: Wenping Zhu, Xinlu Wang, Liyan Dai, Weijie Yang, Yanxia Li, Ruilan Liu

  KEYWORDS: Dumbbell Probe, DNA Ligase, SYBR Green I, DNA Ligation Reaction, Exonuclease

  JOURNAL NAME: Open Access Library Journal, Vol.8 No.10, October 9, 2021

  ABSTRACT: Based on the principle that DNA ligation reaction mediates hydrolysis of the dumbbell probe (DP), a novel label-free strategy for sensitive detection of DNA ligase was developed. DNA ligase ligated the nick on DP to generate a fully closed dumbbell DNA structure, which could prevent the hydrolysis of exonuclease, after combining with SYBR Green I (SG I), a strong fluorescent signal was obtained. In the absence of DNA ligase, DP was hydrolyzed into single nucleotides by exonuclease, resulting in a rather weak signal. The linear range of this method for detecting T4 DNA ligase is 0.00004 - 0.004 U/μL, and the detection limit is 0.00003 U/μL. The proposed strategy is sensitive, inexpensive and easy to operate, which may offer an effective tool for further applications in new drug screening.