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Wargo Rub, A.M., Sandlord, B.P., Gilbreath, L.G., Meyers, M.S., Peterson, M.E., Charlton, L.L., Smith, S.G. and Matthews, G.M. (2012) Comparative Performance of Acoustic Tagged and Passive Integrated Transponder Tagged Juvenile Chinook Salmon in the Columbia and Snake Rivers, 2008. Fish Ecology Division, Northwest Fisheries Science Center, National Marine Fisheries Service, Seattle.

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• TITLE: Novel Surgical Technique for Acoustic Transmitter Insertion in Rainbow Trout Reduces the Need for Surgical Training

  AUTHORS: Nathan Huysman, Ashley Kelican, Lauren Van Rysselberge, Jeremy Kientz, Jill M. Voorhees, Michael E. Barnes

  KEYWORDS: Surgery, Acoustic Transmitter, Rainbow Trout, Oncorhynchus mykiss

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.12 No.9, September 28, 2021

  ABSTRACT: The implantation of acoustic transmitters into the peritoneal cavity of fish is typically performed by experienced surgeons. This study compared the effectiveness of an experienced and inexperienced surgeon performing two different types of transmitter insertion techniques on rainbow trout (Oncorhynchus mykiss). The methods used were either a traditional sutured ventral incision or a novel unsutured lateral incision. There was no significant difference in surgical duration, tag retention, or fish survival between the experienced and naïve surgeon. The time to complete surgical wound closure was not significantly different between the fish operated on by an experienced or naïve surgeon, and surgeon experience had no significant effect on the duration of inflammation. However, there were significant differences between the two surgical techniques, regardless of surgeon experience. The ventral incision with sutures method took approximately three times longer to complete than the lateral incision-only method. However, complete wound closure was significantly faster in the sutured ventral incision treatment compared to the unsutured lateral treatment. Post-surgery inflammation was over seven times longer in the fish with sutures compared to those only receiving a lateral incision. Tag retention was not significantly different between the two surgical methods. The results of this study indicate that the unsutured lateral surgical technique used in this experiment can be completed with minimal training and surgical experience in contrast to the standard technique requiring sutures. These results also provide additional support to the use of sutureless surgical techniques for acoustic transmitter implantation.