TITLE:
In Vitro Evaluation of Bacterial Adhesion to Dental and Stainless-Steel Surfaces
AUTHORS:
A. El Aouame, F. El Quars, Z. Bentahar, K. Zerouali, M. Sidqui
KEYWORDS:
Bacterial Adhesion, Mechanism, Staphylococcus aureus Méti, Tooth Surface, Stainless Steel
JOURNAL NAME:
Open Journal of Medical Microbiology,
Vol.11 No.3,
September
14,
2021
ABSTRACT: This study aimed to describe the factors associated
with biofilms formation in dental pathology by comparison of bacterial growth
on dental and stainless-steel surfaces. We studied in
vitro the behavior of Staphylococcus aureus Métis in order to
observe the capacity of adhesion, to evaluate quantitatively the potential of
proliferation and to compare the behavior of this germ in contact with the two
surfaces. The
biomaterials used were cylinders in Stainless steel (AISI 316L), dental
fragments and stainless-steel fragments, all were disinfected for 15 minutes
and then sterilized in a wet autoclave at 120˚C for 30 min. Macroscopic observation with a binocular magnifier of
bacterial proliferation was carried out regularly after 6 h and 24 h of
incubation. Observation by optical microscope based on GRAM staining made it
possible to visualize the presence or absence of bacteria and to differentiate
them. The adhesion of Staphylococcus aureus Méti S on dental fragments
was compared to the one obtained on stainless
steel fragments. We also carried a Bacterial count by optical dosing. The results show that the
ability of this germ to colonize and develop biofilms on surfaces depends
mainly on the characteristics of the surface. Rough surfaces as dental surface
are more likely to developing biofilms than smooth surfaces like
stainless-steel surface.