TITLE:
Is There Any Role for Calcium Ionophore in ICSI Cycles for Cases of Non Obstructive Azoospermia?
AUTHORS:
Mohamed Elmahdy, Yasser Oreif, Soad Musleh, Emadeldin Khalifa
KEYWORDS:
Fertilization Failure, ICSI, Azosperemia, Ca Ionophore
JOURNAL NAME:
Open Journal of Obstetrics and Gynecology,
Vol.11 No.8,
August
23,
2021
ABSTRACT: Objectives: Evaluate the effect of artificial oocyte activation (AOA) using calcium
ionophore (A23187) on the rate of fertilization and cleavage of embryos in
surgically retrieved sperm of patients with non-obstructive azoospermia
undergoing intracytoplasmic sperm injection (ICSI). Study design: This study was conducted on 60 infertile couples undergoing ICSI cycles
as a randomized controlled parallel group’s experimental study in a private IVF
center in Egypt from January 2018 to July 2019. ICSI cycles were divided into
two groups: Group A: includes 30 ICSI
patients with surgically retrieved sperms of non-obstructive azoospermia
treated with calcium ionophore (A23187). Group C/Control: includes 30 ICSI
patients with surgically retrieved sperms of non-obstructive azoospermia
non-treated with calcium ionophore (A23187). Results: There was
no statistical difference between both groups regarding the fertilization rate
(p = 0.853). There was no statistical difference between them regarding
implantation rate (p = 0.237). The percentage
of Class A embryos in the ca ionophore group was 81.7%, while it was 82.8% in
the control group. There was insignificant difference between them (p =
0.782). There was no statistical significant difference between the two groups
regarding the clinical pregnancy rate, it was
(56.7%) in the ca ionophore group while it was (53.3%) in the control group. Conclusion: AOA by Ca2++ ionophore
didn’t improve the outcome of ICSI cycle in cases of non obstructive
azoospermia in terms of fertilization, implantation and pregnancy rate.