TITLE:
Effect of Flaxseed on Bile Tolerances of Lactobacillus acidophilus, Lactobacillus bulgaricus, and Streptococcus thermophilus
AUTHORS:
Meghana Theegala, René Adalberto Chigüila Arévalo, Valerie Viana, Douglas Olson, Kayanush Aryana
KEYWORDS:
Flaxseed, Bile tolerance, Lactobacillus acidophilus, Lactobacillus bulgaricus, Streptococcus thermophilus, Probiotic
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.12 No.7,
July
13,
2021
ABSTRACT: Consumption of flaxseed provides health
benefits. Bile tolerance allows survival of probiotics in the intestinal
tract. The objective was to determine whether or not flaxseed enhances bile
tolerance of Lactobacillus acidophilus (L. acidophilus) LA-K, Lactobacillus
delbruekii ssp. bulgaricus (L. bulgaricus) LB-12, and Streptococcus salivarius ssp. thermophilus (S. thermophilus) ST-M5. Control and experimental (62
g flaxseed/L) broths containing 0.3% oxgall were prepared for each culture,
sterilized, cooled, inoculated, and plated for 8 h. Growth of each microorganism in both the control and experimental
broths was evaluated by the slope of the regression line of its log count
versus time after inoculation. Flaxseed significantly enhanced growth of L. acidophilus but not L. bulgaricus and S. thermophilus over 8 h compared to its corresponding control.
Therefore, flaxseed improved the bile tolerance of L. acidophilus but not of S. thermophilus and L. bulgaricus.