TITLE:
High Expression of hsMAD2 in the Villi of Spontaneously Aborted Embryo with Chromosomal Abnormality
AUTHORS:
Zaochang Wang, Junjie Hu, Wang He, Humin Gong, Weihua Xu, Zhichao Ma, Xinping Chen
KEYWORDS:
Spontaneous Abortion, Embryonic Villi, Chromosomal Abnormality, hsMAD2
JOURNAL NAME:
Open Journal of Obstetrics and Gynecology,
Vol.11 No.6,
June
18,
2021
ABSTRACT: Objective: To investigate the changes of hsMAD2 protein and
gene expression levels during chromosome segregation of human embryos. Method: The embryos of spontaneous abortion were collected
in our hospital from 2009 to 2013, the chromosomal numbers of the embryonic
villi were subsequently detected by fluorescence in situ hybridization (FISH).
The patients were then divided into the normal and abnormal groups based on the
chromosome number. The hsMAD2 protein and gene expression levels in the villi
tissues of the two embryo groups were detected by western blotting and qRT-PCR,
respectively. The hsMAD2 protein and gene levels in the embryonic villus tissue
of the patient were detected. Results: From 2009 to 2013, we collected 50 embryos from
spontaneous abortion patients. The chromosome abnormality and no abnormality
were 36 cases (abnormal number of 28 cases (56.0%) and chimerism in 8 cases
(16.0%)) and 14 cases (28.0%), respectively. The
expression of hsmad2 protein and its gene in the villi of spontaneously aborted
embryo with chromosomal abnormality in the abnormal group was significantly
higher than that in those without chromosomal abnormalities (0.88 ± 0.20 vs
0.61 ± 0.19, P Conclusion: Abnormal number of chromosomes is closely related
to spontaneous abortion Linked, hsMAD2 factor has a card effect on the cell
cycle, can block the mitotic process of cells, and play an important role in
maintaining the normal separation and stability of chromosomes.