TITLE:
Comparison of Hematological Parameters and Critical Value Analysis in the Prevention and Control of Thalassaemia in Hainan Li and Han Nationalities
AUTHORS:
Junjie Hu, Xinping Chen, Xiaojuan Li, Weihua Xu, Shengmiao Fu
KEYWORDS:
Blood Cell Analysis, Hemoglobin Electrophoresis, Gene Detection, ROC Curve
JOURNAL NAME:
Open Journal of Blood Diseases,
Vol.11 No.2,
May
28,
2021
ABSTRACT: Objective: To compare the distribution of “mean corpuscular
hemoglobin”-MCV, “mean corpuscular volume”-MCH, “hemoglobin”-HGB, “hemoglobin
A”-HbA and “hemoglobin A2”-HbA2 in α and β thalassemia hematology
screening between Li and Han nationality, and analyze the best diagnostic cut-off
value. Methods: Select 7816 middle school students from Li nationality area as the
research object, collect peripheral blood for blood cell analysis, hemoglobin
electrophoresis and thalassaemia gene detection, and compare the difference in
hematological parameters of common thalassemia genotype between Li and Han
nationalities. Taking the genetic test results as the gold standard, construct
the receiver operator characteristic curve (ROC curve) of relevant hematology
parameters, calculate the Youden index and take its maximum diagnostic cut-off
point as the best critical value. Results: Comparison of hematological parameters of common
thalassemia genotypes showed that the average value of MCH and MCV of -α3.7/-α4.2 type in Li nationality was lower than that of Han nationality,
and the average value of HbA2 of CD41-42/βN
type was higher than that of Han nationality, there was no significant
difference among other genotypes. ROC curve analysis shows that the MCH, MCV,
and HGB values have poor diagnostic efficiency for thalassaemia, HbA has a slightly better
diagnostic efficiency for α thalassaemia, and the optimal cut-off values of HbA for Li and Han nationalities are 96.95% and 97.75%, respectively; HbA2 has better
screening efficiency for β-thalassemia,
and the optimal cut-off values of HbA2 for Li and Han nationalities are 4.20%
and 3.45% respectively. Conclusion: In the prevention and control screening of thalassaemia
in the Li and Han nationalities, hemoglobin electrophoresis technology has a
better diagnostic efficiency.