TITLE:
Anomeric Proton and Carbon (H1-C1) NMR Chemical Shifts of Antigenic Mannans Obtained from Pathogenic Yeast Candida tropicalis
AUTHORS:
Takuya Kuraoka, Takayoshi Yamada, Yuki Takatsutsumi, Yukiko Ogawa, Hidemitsu Kobayashi
KEYWORDS:
Candida tropicalis, Cell Wall Mannan, Comb-Like Structure, 1H-13C Correlation Spectroscopy, Anomeric Carbon Chemical Shift, α-1, 3-Linked Mannose
JOURNAL NAME:
Advances in Microbiology,
Vol.11 No.5,
May
26,
2021
ABSTRACT: On two dimensional maps of 1H-13C correlation spectroscopy (H-C COSY) analysis for the mannan of Candida tropicalis, nine cross peaks of anomeric proton and carbon were useful for the purpose of obtaining information on the chemical structure of this molecule. Namely, the mannans was comb-like structure constructed with the linear α-1,6-linked polymannnosyl backbone and several oligomannnosyl side chains composed of α-1,2-, α-1,3-, and β-1,2-linkages. Therefore, in the structural investigation of comb-like mannan, two-dimensional H-C COSY analysis is as useful as two-dimensional nuclear Hartmann-Hahn (HOHAHA) analysis.