TITLE:
The Response of Cockerel Semen Kinematic Parameters LIN, STR, WOB, ALH and BCF to Induced Oxidative Stress
AUTHORS:
Cyril Mpho Pilane, Mohleko Helen Mapeka
KEYWORDS:
Cockerel, Oxidative Stress, Kinematic Parameters, Semen
JOURNAL NAME:
Open Journal of Animal Sciences,
Vol.11 No.2,
April
30,
2021
ABSTRACT: Computer-assisted sperm
class analyser (CASA) analysis of avian semen following cryopreservation
indicates that their semen motility and viability parameters become
compromised, due in part to oxidative stress. To mimic these observations we
have treated cockerel semen with an oxidative stress inducing agent, namely
hydrogen peroxide (H2O2) and
monitored the motility, kinematic and viability parameters over time. Briefly,
five healthy and fertile South African Venda cockerels were selected and their
semen was collected using the abdominal massage technique. The semen was then
treated with H2O2 at 0 µM,
5 µM, 50 µM and 200 µM concentrations for 0, 3, 16
and 24 hrs. The semen motility, kinematic and viability parameters were then
determined using the CASA system while the viability was determined using the
SYBR-14/PI staining. The Pearson’s correlation coefficient was determined to
test the relationships between the levels of induced oxidative stress, period
of exposure to oxidative stress inducing agent and the motility plus kinematic
parameters. Our data revealed that in raw cockerel semen, there was high and
positive correlations between total motility (TM), progressive motility (PM),
rapid (RAP), curvilinear velocity (VCL), straight line velocity (VSL) and
average path velocity (VAP) while the kinematic parameters LIN, STR, WOB, ALH
and BCF had low or negative correlations with them. Furthermore, TM, PM, RAP,
VCL and VSL remained highly and positively correlated with the induced
oxidative stress and also, linearity (LIN), straightness (STR), wobble (WOB),
amplitude of lateral head displacement (ALH) and beat cross frequency (BCF)
remained negatively correlated with the induced oxidative stress, after 3 hrs.
After 24 hrs, TM, PM, RAP, VCL, VSL, VAP and ALH, became negatively correlated
with the induced oxidative stress while LIN, STR, WOB and BCF became positively
correlated with the induced oxidative stress. Conversely, when the H2O2
concentration used was correlated with motility and kinematic parameters over
time, TM, PM, RAP, VCL, VSL, VAP became negatively correlated with oxidative
stress while LIN, STR, WOB, ALH and BCF show negative or low correlations with
the induced oxidative stress. This data indicates that LIN, STR, WOB, BCF and
to some extend ALH, reveal the least correlations with the induced oxidative
stress under persistent oxidative stress conditions in cockerel semen. In
conclusion, cockerel semen, like buck semen, does not easily succumb to
oxidative stress since the raw semen correlations of CASA analysed parameters
are comparable to these observed after 3 hrs of H2O2
treatment. In addition, the oxidative stress levels tolerated by cockerel semen
should not 5 µM H2O2
oxidative stress levels. Lastly, lack of correlation between LIN, STR, WOB, BCF
and ALH and induced oxidative stress can be used in cockerel semen to show
intolerable cryopreservation conditions.