TITLE:
Comparison of Two Enzyme Immunoassays and Four Lysate Antigens for the Detection of Antibody in Canine Blastomycosis
AUTHORS:
Matthew Day, Arin Esterbrook, Ignatius Bisharat, Abdullah Saleh Albqomi, Bryn Kennell, Russ Manteca, Heaton Oakes, Geoffrey M. Scalarone, Gene M. Scalarone
KEYWORDS:
Blastomyces lysate Antigens, Antibody Detection, ELISA Methods, Canine Blastomycosis, Blastomyces dermatitidis, Blastomyces gilchristii
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.11 No.4,
April
28,
2021
ABSTRACT: Blastomycosis,
the systemic fungal disease of humans and animals caused by Blastomyces
dermatitidis and the cryptic species Blastomyces gilchristii, is often misdiagnosed as a
bacterial or viral pulmonary disease. Therefore, the development of improved
immunodiagnostic assays for this disease has been the primary focus of research
in our laboratory. The present study was designed to evaluate four Blastomyces yeast-phase lysate
antigenic preparations (human, 597, Eagle River, WI; dog, ERC-2, WI; Human,
B5927, Mountain Iron, MN; soil, 85, Georgia, ATCC 56920) for their ability to
detect antibody in 48 serum specimens from dogs with diagnosed blastomycosis
using an indirect ELISA (STD) compared to a biotin-streptavidin ELISA (B-SA).
All four lysate antigens were able to detect antibodies in the specimens with mean absorbance values
ranging from 0.930 (B5927) to 1.142 (ERC-2) with the STD ELSA and from 1.395
(B5927) to 1.775 (85) with the B-SA ELISA. The results indicated that both
ELISA methods could be utilized for antibody detection, but the B-SA ELISA
exhibited greater sensitivity than the STD ELISA with all four of the lysates.