TITLE:
Impact of Yupingfengsan Oral Liquid on the Expression of IL-4, IFN-γ and NF-κB of Allergic Rhinitis Mice
AUTHORS:
Benyuan Chen, Chenggang Mao, Yating Wan, Lijia Wan
KEYWORDS:
Allergic Rhinitis (AR), Yupingfeng Powder Oral Solution, Nuclear Factor-κB (NF-κB), Interleukin-4 (IL-4), γ-Interferon (INF-γ)
JOURNAL NAME:
Yangtze Medicine,
Vol.5 No.2,
April
15,
2021
ABSTRACT: To observe the impact of Yupingfengsan Oral Liquid on the expression of IL-4 and IFN-γ in AR mice’s serum and expression level of nuclear factor—κB (NF-κB) protein and gene in nasal mucosa. Method: Forty BALB/c mice were randomly divided into the normal group, model group, Yupingfengsan Oral Liquid group (6 g/kg) and Loratadine group, with 10 mice per group. AR mice model was established by OVA, and IL-4 and IFN-γ contents can be measured with ELISA. The morphological changes of nasal mucosa were observed by hematoxylin eosin (HE) staining and NF-κB expression in the nasal mucosa of mice was tested with Real-Time PCR and Western blot. Results: Compared with the model group, the nasal symptoms in the Yupingfengsan Oral Liquid group and Loratadine group were obviously relieved. HE staining showed that there was a little inflammatory cell infiltration in the nasal mucosa of Yupingfengsan Oral Liquid group and Loratadine group and it was significantly reduced when compared with the model group. IL-4 level in the serum and expression of NF-κB protein and gene in the nasal mucosa was consistent and it was decreased when compared with the model group (P γ level in the serum was increased (P Conclusion: Yupingfengsan Oral Liquid can improve the clinical symptoms and histopathological manifestations of AR mice sensitized by OVA, inhibit the NF-κB expression, balance the percentage of Th1/Th2 cells, increase the IFN-γ level in the serum and decrease the IL-4 level.