TITLE:
A Study of Prevalence and Pathogenic Activity of Bacteria in the Air of Dhaka City and Their Antimicrobial Resistance Pattern
AUTHORS:
Shaeri Nawar, Maliha Tabassum Rashid, Akash Ahmed, M. Mahboob Hossain, Asma Binte Afzal
KEYWORDS:
16S rRNA Sequencing, Pathogenecity, Multi-Drug Resistant
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.11 No.2,
April
16,
2021
ABSTRACT: Bangladesh composes the most polluted air with Dhaka securing the top position. The purpose of the study is the enumeration of the prevalence of pathogenic bacteria in Dhaka city’s air and their antibiotic susceptibility to the common antibiotics. For the sample collection, different selective media was exposed in air where the highest and lowest CFU was 137 and 1 respectively. Pathogens were screened through Hemolysis, DNase and Coagulase test and identified by 16s rRNA sequencing followed by antibiotic susceptibility test. 16s rRNA sequencing revealed that the organisms were Bacillus altitudinis strain 41KF2bT.28, Bacillus licheniformis strain QMA46-2, Bacillus altitudinis, Bacillus pumilis strain BJ-DEBCR-34, Staphylococcus aureus strain TPS3156, Bacillus sp CO16, Pseudomonas sp strain 96LC22 and Shigella dysenteriae strain ATCC 13313. Shigella dysenteriae, Staphylococcus aureus were 81.81% and 54.54% resistant to the antibiotics. Whole-genome sequencing would help to observe mutations in the traits as changes in hemolytic activity were found during pathogenecity tests.