TITLE:
Clinical Evaluation after Introduction of Stem Cell Supernatant Biocell Shot 65 by Electroporation
AUTHORS:
Ryusuke Suzuki, Harue Suzuki, Maki Yokogawa, Junichi Motohashi
KEYWORDS:
Clinical Evaluation, Stem Cell, Skin Treatment
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.11 No.1,
March
30,
2021
ABSTRACT: Objectives: The
objective of this study is clinical evaluation after introduction of stem cell
supernatant Biocell Shot 65 by electroporation. Background: There is a clinical report of psoriasis treatment by umbilical cord
blood-derived stem cells, in which there was a beneficial result. These
clinical results are the result of cord blood-derived cell growth factors. The
umbilical cord blood-derived stem cell culture supernatant contains a high
amount of TGF-β, PDGA, etc., and
promotes wound healing. In addition, we suppose that the supernatant has the esthetic effect of improving skin
condition, thus, we planned to make a clinical evaluation. However, it
is difficult to set judgement criteria, and human judgement tends to be
subjective. Therefore, in this study, as a new clinical evaluator, we used an
AI skin diagnostic device with learning function. Methods: Stem cell
culture supernatant Biocell Shot 65 was used as an introduction solution. After
it was applied to the full faces of four patients, electroporation with BeBe
pinocchio DM-5 SUPER DX Plus promoted infiltration deep in the keratinized
layer. The patients were all healthy women
in their forties. This procedure was performed every two weeks for three
months. Wrinkles, fine lines around eyes, stains, pores, and 4V, which is considered as one diagnostic criteria,
were evaluated at baseline and after each procedure with the skin
diagnostic device HiMirror-Professional. In this study, macroscopic findings
were not examined. Results: In all four patients, 4V did not change
remarkably. Patient 1 showed improvement after the procedure in wrinkles, fine
lines around eyes, stains, and pores. Patient 2 revealed improvement only in
fine lines around eyes, but did not show improvement in other items. Patient 3
improved in wrinkles, stains, and pores only after the second procedure, but
did not show improvement in the other procedures. Patient 4 improved in all
items except 4V after the fourth procedure. In addition, when the mean values
of all the patients were plotted and compared before and after each procedure,
there was a trend toward improvements after the fifth procedure. Conclusion: In addition to application of supernatant culture to the skin, introduction of
the solution to the skin by electroporation might improve stains, wrinkles, and
pores. Our evaluation was performed with a facial diagnostic device three
months after the start of the procedure.