TITLE:
Antimicrobial and Antioxidant Activities of Methanolic Extract and Fractions of Epilobium roseum (Schreb.) against Bacterial Strains
AUTHORS:
Muhammad Ikram, Niaz Ali, Gul Jan, Farzana Gul Jan, Muhammad Romman, Muhammad Ishaq, Yasir Islam, Naeem Khan
KEYWORDS:
Methanolic Extract, Epilobium roseum, DPPH, n-Hexan, Antioxidant Activity
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.12 No.3,
March
9,
2021
ABSTRACT: Present study aims to evaluate antimicrobial and antioxidant activities
of the crude methanolic extract and different fractions of the Epilobium roseum (Schreb). The extract
and fractions were used against pathogenic bacteria (Bacillus subtilis, Bacillus atrophaeus, Klebsiella pneumonia and Pseudomonas aeruginosa)
and fungal strains (Aspergillus niger and Aspergillus flavus). The methanolic extract and their sub fraction
n-hexane showed a prominent inhibition zone against all bacterial strains but
inactive against fungal strains. The various
extracts of Epilobium roseum (Schreb) from various parts were tested for their antioxidant activity
by 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) assay. The IC50 of the stem and root of methanolic extract
and their sub fraction n-hexane showed best activity ranged between 22.73 ±
6.92, 21.49 ± 6.26 and 14.94 ± 3.54, 13.92 ± 1.04 μg/ml compared to another fraction.
The results support that Epilobium roseum can be used as antimicrobial and antioxidant agents. The
results support the present study that Epilobium roseum (Schreb) has a potential source of natural
antibacterial, antifungal and antioxidant potentials.