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Hanai, Y., Matsuo, K., Kosugi, T., Kusano, A., Ohashi, H., Kimura, I., Hirayama, S., Nanjo, Y., Ishii, Y., Sato, T., Miyazaki, T., Nishizawa, K. and Yoshio, T. (2018) Rapid, Simple, and Clinically Applicable High-Performance Liquid Chromatography Method for Clinical Determination of Plasma Colistin Concentrations. Journal of Pharmaceutical Health Care and Sciences, 4, Article No. 22.
https://link.springer.com/article/10.1186/s40780-018-0119-x
https://doi.org/10.1186/s40780-018-0119-x

has been cited by the following article:

• TITLE: LC-MS/MS Method for Determination of Colistin in Human Plasma: Validation and Stability Studies

  AUTHORS: Nada H. Binhashim, Syed N. Alvi, Muhammad M. Hammami

  KEYWORDS: Colistin, Clarithromycin, Human Plasma, LC-MS/MS

  JOURNAL NAME: International Journal of Analytical Mass Spectrometry and Chromatography, Vol.9 No.1, March 2, 2021

  ABSTRACT: A simple and reliable high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for the determination of colistin A and colistin B in human plasma was developed and validated. Clarithromycin was used as an internal standard (IS). Plasma extraction was performed using C-18 cartridges and methanol containing 0.1% formic acid. Analysis was performed using Atlantis dC18 (2.1 × 100 mm, 3 μm) column at room temperature and a mobile phase of 0.2% formic acid in acetonitrile and water (50:50, v:v), delivered at a flow rate of 0.2 ml/minute. Eluent was detected in the positive ion mode using electrospray ionization at the following transitions of mass to charge (m/z): colistin A, 585.6 → 101.4; colistin B, 578.7 → 101.3; and IS, 748.6 → 158.4. No interference by components of blank plasma or commonly used drugs was observed. The relationship between colistin A colistin B concentrations and their corresponding peak height ratios to the IS was linear over the range of 0.05 - 10 μg/ml. Inter-day coefficient of variation and bias were, respectively, ≤11.5% and −3.0 to 6.0 for colistin A and ≤9.9 and −4.7 to 3.0 for colistin B. Mean extraction recovery of colistin A, colistin B, and the IS were 97%, 94%, and 97%, respectively. The method was applied to assess the stability of colistin A and colistin B in processed samples (24 hr. at room temperature, 48 hours at −20°C) and unprocessed samples (24 hr. at room temperature, 8 weeks at −20°C) and after three cycles of freeze and thaw found to be ≥87%.