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Bouizgarne, B., Brault, M., Pennarun, A.M., Rona, J.P., Ouhdouch, Y., El Hadrami, I. and Bouteau, F. (2004) Electrophysiological responses to fusaric acid of root hairs from seedlings of date palm-susceptible and -resis- tant to Fusarium oxysporum f. sp. Albedinis. Journal of Phytopathology, 152, 321-324.

has been cited by the following article:

• TITLE: The effects of neem [Azadirachta indica A. Juss (meliaceae)] oil on Fusarium oxysporum f. sp. medicagenis and Fusarium subglutinans and the production of fusaric acid toxin

  AUTHORS: Marcia Regina Ferreira Geraldo, Carla Cristina Arroteia, Carlos Kemmelmeier

  KEYWORDS: Fusarium; Azadirachta Indica; Fusaric Acid; Antifungal Activity

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.1 No.1, April 22, 2010

  ABSTRACT: Fungi of the genus Fusarium are well known plant pathogens, cause several vascular diseases and are producers of toxins. In vitro assays evaluated the effects of Neem (Azadirachta indica) oil on the diameter of colonies, dry weight, spore production, spore viability and production of Fusaric Acid toxin on Fusarium oxysporum f. sp. medicagenis and Fusarium subglutinans isolates. Effects of Neem oil were analyzed at concentrations 0.25%, 0.5% and 1% in Czapek Yeast Agar medium. The production of Fu- saric acid was determined by Thin Layer Chromatography and quantified by UV spectrophotometry. Neem oil showed inhibitory effects on the isolates tested, although they varied according to type of isolate and oil concentration. Neem oil was efficient in reducing the colonies’ diameter and dry weight in concentration-dependent manner. Neem oil was efficacious at higher concentration in the decrease of sporulation. Spore germination was affected by Neem oil when the spore was grown in Neem-contained medium as when the spore emerged from a culture in a Neem medium. Neem oil decreased and even inhibited the production of Fusaric acid by the assayed isolates. Since these isolates are plant pathogens and producers of Fusaric acid, Neem oil may be introduced as an integral item in the management of host plants.