TITLE:
Gene Expression Profiling of the Mouse Pancreas during the Secondary Transition in the Organogenesis of the Pancreatic Gland*
AUTHORS:
Stefanie J. Willmann
KEYWORDS:
Gene Expression Profile, Pancreas Organogenesis, Single-Nucleotide Polymorphism, Type 1 Diabetes, Type 2 Diabetes
JOURNAL NAME:
Journal of Diabetes Mellitus,
Vol.11 No.1,
February
3,
2021
ABSTRACT: Diabetes mellitus is a chronic disease that
impacts the homeostasis of blood sugar levels caused by loss or defect of
insulin-producing β-cells in the Islets of Langerhans. Type 1 diabetes (T1D) is caused by auto-immune mediateddestruction of
β-cells, whereas in T2D, insulin is produced but used inefficiently. T2D accounts for 90% of people with diabetes worldwide (WHO 1999) and is the fastest increasing disease worldwide (https://diabetesatlas.org/en/). For an improved understanding of the pathomechanism of
diabetes, profound knowledge of pancreas organogenesis and the associated gene
regulatory networks is required. Therefore, we dissected and profiled the
pancreatic endodermal and non-endodermal compartment between the embryonic
stages (E) 12.5 and E 15.5 when progenitor cells commit to their different
pancreatic lineages. Our associated study mined the global mRNA expression
profile to increase the understanding of the secondary transition,
endodermal-non-endodermal tissue interaction, and diabetic-related gene
regulation. Furthermore, we validated 635 regulated pancreatic genes using the
publicly available GenePaint.org,
respective gp3.mpg.de to evaluate genes associated with genetic variants in
Single-nucleotide polymorphism (SNP) related to T2D.