TITLE:
Analysis of Two-Dimensional Gel Electrophoresis Map of Methicillin-Resistant Staphylococcus aureus Treated with Acetone Extract from Canarium odontophyllum Miq. Leaves
AUTHORS:
Nur Amira Mohd Shamsuddin, Dayang Fredalina Basri, Noraziah M. Zin, Ahmad Raha Raus, Nur Faizah Abu Bakar
KEYWORDS:
Canarium odontophyllum, MRSA, 2-DE, IEF, SDS-PAGE Protein Expression Profile
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.12 No.1,
January
22,
2021
ABSTRACT: Methicillin-resistant Staphylococcus aureus (MRSA) infection is a global health concern
that has caused severe health threats over the past decade. Leaves extract of C. odontophyllum has been proven
previously as an anti MRSA agent. Proteomics provide
a technique that used to analyze the
differential of protein expression profile between untreated and treated MRSA
with subinhibitory concentrations of acetone extract from C. odontophyllum leaves. This
study aims to determine the optimum parameter for analysis of protein
expression profile using two-dimension gels electrophoresis (2-DE) for MRSA
protein after treatment with acetone extract from C. odontophyllum leaves. Comparison of the Protein
Expression Profile (PEP) between the untreated and treated MRSA was analyzed
using PDQuest software. The optimum condition for MRSA protein treated with
acetone extract from C. odontophyllum leaves to produce the best resolution with greater spot distribution was as
follows: 100 μg volume of MRSA protein that loaded after passive rehydration
then was run until reaching 25 kVrhs
during IEF using 17 cm IPG strip within ranges of pH 4 - 7. Analysis of protein
expression from the 2-DE gel map shows that 9 protein spots up-regulated and 41
protein spots were down-regulated with more than 2-fold differences (p C. odontophyllum leave may provide an
insight into the antimicrobial mechanism, which could lead to the
identification of target protein for future novel therapeutic development
against MRSA infections.