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Cockerill, F.R., Wiker, M.A., Alder, J., Dudley, M.N., Eliopoulos, G.M., Ferraro, M. J., Hardy, D.J., Hecht, D.W., Hindler, J.A., Patel, J.B., Powell, M., Swenson, J.M., Thomson, R.B., Traczewski, M.M., Turnidge, J.D. and Weinstein, M.P. (2018) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically.

has been cited by the following article:

• TITLE: Identity and Safety of a Novel Aurantiochytrium sp. for Terrestrial Heterotrophic Docosahexaenoic Acid Production

  AUTHORS: Andrew Norman William Bell, Colm Anthony Moran

  KEYWORDS: Thraustochytrid, Docosahexaenoic Acid, Fatty Acids, Aurantiochytrium limacinum, Minimum Inhibitory Concentration, Antimicrobial Resistance

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.11 No.12, December 31, 2020

  ABSTRACT: The objective of the studies in this paper was to expand on the published toxicological assessment of Aurantiochytrium limacinum (AURA) with further strain characterization and to investigate the potential for the biomass or extracted oil to have antimicrobial properties or undesirable substances. AURA is being investigated as a novel source of the omega-3 long-chain polyunsaturated fatty acid docosahexaenoic acid (DHA) for enriching foods of animal origin by means of feed supplementation. In the first studies, we provided the 18S rRNA identification of the novel marine isolated thraustochytrid, established the nutritional composition of AURA biomass for application as a food or feed ingredient including proximate analysis and fatty acid profiling, and confirmed the DHA production potential of the strain. We determined through minimum inhibitory concentration (MIC) analysis that the unextracted AURA biomass was safe, showing no antimicrobial influence and no evidence of any deleterious effects of this product or its extracts at concentrations up to 1% w/w on the reference human intestinal bacteria tested. This would indicate that AURA should not stimulate selective pressure on the commensal microbiota and is therefore unlikely to aid development of antimicrobial resistance and the concomitant harm to humans and animals. Further analysis revealed that the AURA biomass produced through industrial heterotrophic fermentation was free from undesirables; toxic marine microalgal metabolites, heavy metals, pesticides, microbial contaminants, and mycotoxins. Including heterotrophically-grown AURA in food or feed, up to 1% w/w, is a safe and environmentally beneficial strategy for DHA supplementation.