TITLE:
Analysis of PTEN Methylation Pattern by Pyrosequencing in Renal Cell Carcinoma
AUTHORS:
Eurico Cleto Ribeiro de Campos, Marcos Gonçalves Adriano Júnior, Marcus Rivabem Winheski, Renan Martins, Ademar Lopes
KEYWORDS:
Methylation, PTEN, Pyrosequencing, Renal Cell Carcinoma
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.11 No.12,
December
15,
2020
ABSTRACT: Objectives: We aimed to determine the frequency of PTEN (phosphatase with tensin
homology deleted in chromosome 10) hypermethylation in renal cell carcinoma
(RCC) and its impact on overall (OS) and disease-free survival (DFS). The
association between PTEN hypermethylation and clinic and pathologic factors was
also analyzed. Materials and Methods: The authors analyzed 137 patients
who had undergone a radical or partial nephrectomy between 1997 and 2011. The
methylation pattern was quantified individually at multiple CpG islands that
are located on the exon 1 of PTEN gene by pyrosequencing. Results: Mean
follow-up was 32.3
months. PTEN hypermethylation was detected with a low frequency (3.6%). There
were no deaths or disease recurrence among patients with PTEN hypermethylation.
Determining the association between PTEN hypermethylation and clinical and
pathological factors was not possible due to the low frequency observed. Conclusion: The
results show that the methylation of promoter CpG sites in PTEN is relatively
infrequent in renal carcinogenesis and has no prognostic impact on survival.