TITLE:
Hepatic Protective Effects of S-Allyl-L-Cysteine (SAC) in Rats with Carbon Tetrachloride (CCl4) Induced Liver Injury
AUTHORS:
Soo-Nyun Choi, H. M. Arif Ullah, Il-Hwa Hong, Jin-Kyu Park, SunYoung Park, Myung-Jin Chung, Ji-Yoon Son, Hyun-Ho Yun, Jae-Hyuk Yim, Seung-Jun Jung, Hae-Young Chung, Kyu-Shik Jeong
KEYWORDS:
S-Allyl-L-Cysteine (SAC), Garlic, Hepatic Protective Effects, Carbon Tetrachloride, Rats, Hepatic Steatosis, Cytochrome P4502E1
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.11 No.12,
December
15,
2020
ABSTRACT: S-allyl-L-cysteine (SAC) is an organosulfur compound derived from aged
garlic extract (AGE). Studies
have reported that AGE possesses bioprotective capacity, including
antidiabetic, antimicrobial, antioxidant, and antitumor effects. The present
study examined the protective effects of SAC against carbon tetrachloride (CCl4)
induced hepatotoxicity in rats. Ten male Wistar rats aged 11 - 12 weeks were
randomly divided into two groups (five rats/group) as control and SAC groups.
All rats had ad libitum access to water, and the SAC
group received water containing SAC intragastrically (200 mg/kg) once daily for
five consecutive weeks. In the fifth experimental week, 50% CCl4 in
olive oil (1 mL/kg) was administered intraperitoneally three times a week to
induce liver injury in both groups. Rats were sacrificed at 24 hours after the
last CCl4 injection, and liver tissues were excised for histopathological,
immunohistochemical and antioxidant analyses. The rats in the SAC group did not
show abnormal behavior, such as decreased water intake or food consumption,
during the experimental period. Body weights in all groups did not change
significantly over the experimental period. Histopathological analysis showed
that the percentage of hepatic steatosis was lower in the SAC group at 12.75% ±
3.74% compared to 24.64% ± 5.29% in the control group (p p 4-induced
liver damage by decreasing hepatic steatosis and reducing CYP2E1 expression in
rats.