TITLE:
Isolation of Filamentous Fungi from the Caatinga Region and Production of Amylolytic Enzymes of Great Industrial Interest
AUTHORS:
Ingrid Cristina Soares Amorim, Gessica Oliveira Marinho, Tarcisio Michael Ferreira Soares de Oliveira, Juan Pedro Bretas Roa, Arlete Barbosa dos Reis, David Lee Nelson, Thiago Machado Pasin, Vivian Machado Benassi
KEYWORDS:
Amylolytic Enzyme, Bioprospecting, Filamentous Fungi, Industrial Interest
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.8 No.11,
November
19,
2020
ABSTRACT: The industrial importance and the high cost of the commercial amylase require the study of microorganisms that produce these enzymes. For this reason, the objective of this work was to isolate filamentous fungi from a region of the caatinga and evaluate their potential for the production of amylase. Four soil samples were collected from a deactivated dump located in the city of Diamantina, MG, in a region of the caatinga. The analysis of amylolytic production in a submerged medium at the ideal temperature of each microorganism was performed using the saccharification method, and the reducing sugars formed were quantified by DNS. Fourteen filamentous fungi were isolated, which had different morphological aspects. Regarding amylase production, a mean activity of 0.477 U·mL-1 was obtained with the isolates I 1.2.1 and I 4.4.1. These results bring important information regarding the biodiversity of the caatinga, in addition to the isolation of microorganisms that can be used as biological machinery to obtain metabolites with high biotechnological and industrial potential.