TITLE:
Development and Validation of Methods for Identification and Quantification of Biological Active Substances in the Fruit of Tetrapleura tetraptera
AUTHORS:
Ebenezer Frimpong, Liliya Logoyda
KEYWORDS:
Biological Active Substances, Tetrapleura tetraptera, SPU
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.11 No.9,
September
27,
2020
ABSTRACT: This study was conducted to identify the biological active substances (BAS) from the fruit of Tetrapleura tetraptera obtained from Ghana. TLC, UV Spectroscopy and differential spectrophotometry methods of analysis were employed in this study. Identified BAS included flavonoids, polysaccharides and amino acids. Following state pharmacopoeia of Ukraine (SPU) requirements to validate the developed techniques were employed in this study, and the following validation properties specificity, linearity, range, precision accuracy (convergence) and robustness was determined. These characteristics did not exceed the eligibility criteria as stated in (SPU) Appendix 2. Thus the technique can be made to the SPU monograph on fruits of Tetrapleura tetraptera.