TITLE:
Assessment of Genetic Variation in Soybean (Glycine max) Accessions from International Gene Pools Using RAPD Markers: Comparison with the ISSR System
AUTHORS:
Kabwe Nkongolo, Sarah Alamri, Paul Michael
KEYWORDS:
Soybean, Glycine max, Genetic Variations, ISSR, RAPD, Molecular Markers
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.11 No.9,
September
24,
2020
ABSTRACT: Soybean (Glycine max) is one of the most important
crops in the world in terms of total production and usage. It is also among the
least diverse species. The main objectives of the present study were to 1)
assess the level of genetic variation among soybean (G. max) accessions from
different countries using Random Amplified Polymorphic DNA (RAPD) markers and
2) compare Inter Simple Sequence Repeats (ISSR) and RAPD marker systems in
detecting polymorphic loci in soybeans (G. max). Genomic DNAs from 108 soybeans
(G. max) accessions from 11 different gene pools were analyzed using
several ISSR and RAPD primers. The average level of polymorphic loci detected
with the RAPD primers was 35%. The soybean accessions from the China,
Netherlands, and Canada gene pools were the least genetically variable with 25%,
26%, and 30% of polymorphic loci, respectively. Accessions from Hungary (43%)
and France (48%) showed the highest level of polymorphism based on the RAPD
analysis. Overall, RAPD data revealed that the accessions from different
countries are closely related with 64% genetic distance values below 0.40. The
levels of polymorphic loci detected with the RAPD and ISSR marker systems were
in general moderate and similar even if they target different regions of the
genome. A combination of different marker systems that include RAPD/ISSR,
microsatellites (SSR), and SNPs should provide the most accurate information on
genetic variation of soybean (G. max) accessions.