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Rejeb, M.N., Laffray, D. and Louguet, P. (1991) Physiologie du caroubier (Ceratonia siliqua L.) en Tunisie. In: Riedacker, A., Dreyer, E., Pafadnam, C. and Joly Hélène, B.G., Eds., Physiologie des arbresetarbustes en zones arides et Semi-Arides, Grouped’Etude de l’Arbre, Paris, 417-426.

has been cited by the following article:

• TITLE: In Vitro Propagation of Mature Carob Trees (Ceratonia siliqua L.) from the Axillary Buds

  AUTHORS: Najat Zouari, Noureddine El Mtili

  KEYWORDS: In Vitro, Ceratonia siliqua, Carob, Propagation, Axillary Bud, Rooting

  JOURNAL NAME: American Journal of Plant Sciences, Vol.11 No.9, September 17, 2020

  ABSTRACT: The influence of tree age and the effect of growth regulators on the micropropagation of the carob (Ceratonia siliqua L.) from the axillary buds of mature trees have been described. Significant differences (P 3. The use of 0.5 mg/l BA and 0.2 mg/l IBA was the most favorable for shoots neoformation. The leafy shoots are propagated in MS medium with BA at a concentration of 1.5 mg/l. The addition of gibberellic acid at 0.2 mg/l in the culture medium allows a good elongation and development of the shoots of the carob. The effect of the age of the plant material used has shown that explants taken from mature carob trees have a low capacity for bud sprouting and shoot proliferation compared to those taken from juvenile trees. Rooting has been successful when the plant material used is taken from young trees on an MS medium containing 2 mg/IBA, with an average number of 3 to 4, roots 1 to 2 cm long, then for the adult material, no rooting was observed. Based on these tests, it appears that micropropagation of the carob from the axillary buds is feasible, but additional work must be done to root this recalcitrant material.