TITLE:
Occurrence of Extended Spectrum Beta Lactamase Encoding Genes among Urinary Pathogenic Escherichia coli and Klebsiella pneumoniae Isolates Obtained from a Tertiary Hospital in Gombe Nigeria
AUTHORS:
Adamu Yarima, Ali Ahmed Haroun, Timothy Bulus, Mohammed M. Manga
KEYWORDS:
ESBL, Double Disk Synergy Test, M-PCR, TEM, SHV, CTX-M Genes, Nigeria
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.8 No.9,
September
9,
2020
ABSTRACT: This study was conducted to assess the occurrence and nature of extended-spectrum beta lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae isolates from patients who presented with urinary tract infection at Federal Teaching Hospital Gombe. Isolates collected were recovered on MacConkey agar at 35°C and were identified as members of Enterobacteriaceae, and further screened for antimicrobial susceptibility and resistance by disc diffusion method. Isolates resistant to oxyimino-cephalosporins were confirmed as ESBL producers using Double Disks Synergy Test (DDST). The study shows 66% resistance to ceftriaxone (30 μg) in K. pneumoniae, which was the highest value recorded and a 51% resistance to cefpodoxime (10 μg) in E. coli. The sensitivity of E. coli and K. pneumoniae isolates to cefpodoxime (10 μg) were 49% and 33.9% respectively. ESBLs were detected among 40% (40/100) of E. coli and 54.13% (59/109) of K. pneumoniae isolates. Molecular characterization of ESBL encoding genes among E. coli isolates using multiplex-PCR showed 10% prevalence of SHV gene and 5% prevalence for CTX-M gene while TEM gene was not detected. In K. pneumoniae isolates, 5% prevalence was recorded for each of the three genes screened. The study revealed a co-occurrence of SHV and CTX-M in 75% of the E. coli and 70% of the K. pneumoniae isolates; the occurrence of all the three genes was seen in 10% and 5% of K. pneumoniae and E. coli respectively. Multiplex-PCR method provided an efficient and rapid detection of ESBL related genes, hence could be used in epidemiological studies among ESBL isolates. Monitoring dissemination and transmissions of ESBL producers are highly recommended for optimum patient care and preventing the spread of multidrug resistant (MDR) pathogens.