TITLE:
Polymorphisms of the Toll-Like Receptor-2 Gene in Patients with Leprosy and Their Healthy Contacts
AUTHORS:
Luana Nepomuceno Gondim Costa Lima, Letícia Siqueira Moura, Jasna Leticia Pinto Paz, Maria do Perpétuo Socorro Amador Silvestre, Daniele Melo Sardinha, Letícia Diogo de Oliveira Moura, Karla Valéria Batista Lima
KEYWORDS:
Polymorphism, Toll-Like Receptors, Leprosy
JOURNAL NAME:
Open Journal of Immunology,
Vol.10 No.3,
August
18,
2020
ABSTRACT: Leprosy is an immunopathology caused by M. leprae; its
evolution depends on immunological and genetic aspects of the host. The
objective was verifying the relationship between SNPs 2029 and 2258 of the
TLR-2 gene and leprosy. Blood samples from 127 individuals were analyzed: 45
patients, being 34 multibacillary (MB) and 11 paucibacillary (PB) and 82
contacts, in the municipalities of the State of Pará-Brazil. SNPs 2029 and 2258
of the TLR-2 gene were genotyped by sequencing on the ABI 3130 Genetic Analyzer
(Applied Biosystems), analyzed using Fisher’s exact test. Distribution of SNP
2029 genotypes: all MB individuals presented the C/C genotype and the mutant
(C/T) genotype was observed in contacts and PB. Alleles: all MB individuals
presented only C allele and the mutant allele (T) was observed in contacts and
PB. SNP 2258 genotypes: 79 contacts had G/G genotype and only 3 had G/A
genotype, the MB group had only G/G genotype and the PB group was predominant
G/G, with only 1 G/A genotype. Alleles: all MB individuals had allele G and the
mutant allele (A) was observed in contacts and PB. The association between the
SNPs and the susceptibility or protection to leprosy was not observed.