TITLE:
Inhibitory Activities of Lactic Acid Bacteria against Multi-Drug Resistant Uropathogenic Staphylococcus saprophyticus Isolated from Symptomatic Women in Lagos, Nigeria
AUTHORS:
Tajudeen Akanji Bamidele, Felix Oluwasegun Alao, Victorious Chisom Ekeh, Muinah Adenike Fowora, Bolanle Alake Adeniyi
KEYWORDS:
Inhibitory, Lactic Acid Bacteria, Resistant, Uropathogenic, Symptomatic
JOURNAL NAME:
Advances in Microbiology,
Vol.10 No.8,
August
14,
2020
ABSTRACT: The uropathogenic Staphylococcus saprophyticus is reported severally to be resistant to the drugs often used
empirically for treatment of urinary tract infections (UTIs). Their ability to
exhibit resistance to multiple drugs is a great deal of threat to successes recorded in the management of UTIs caused
by this pathogen. Lactic acid bacteria (LAB) have been demonstrated to exhibit
antimicrobial activities but studies about
their prospect against multi-drug resistant S. saprophyticus are
quite few. This study therefore investigated activities of LAB against the multi-drug resistant S. saprophyticus recovered
from urine samples of symptomatic women. The
three different species of LAB (Lactobacillus fermentum BTA 62, Lactobacillus johnsonii BTA 86 and Weissella confusa BTA 40) previously isolated and identified by 16S
rRNA sequencing were
selected based on their history of antimicrobial activities. Their metabolites were employed in the antagonistic assays
against six (6) multi-drug resistant
test pathogens recovered from urine samples of symptomatic, non-pregnant
women attending clinics in Lagos, Nigeria and the control (S. saprophyticus subs
bovis strain DSM 18669) following standard procedures. The pathogens showed resistances to almost all the antibiotics except levofloxacin, ciprofloxacin, imipenem while
the control showed resistance to three. The LAB, L. fermentum inhibited five (83.3%) of the pathogens with zone diameter of 12 - 17
mm, followed by W. confusa inhibiting three (50%) with 15 -
17 mm. Lactobacillus johnsonii on the other hand, inhibited a pathogen and the control with zones of 13
mm and 14 mm respectively. In conclusion, the extracted metabolites of LAB
inhibited the growth of multi-drug resistant clinical isolates of uropathogenic S. saprophyticus and may therefore be potent alternatives to antibiotics.