TITLE:
Effects of 5-Aminolevulinic Acid (ALA) on Zinnia hybrida Growth and Phytoremediation Effects in Oil-Contaminated Soil
AUTHORS:
Takamitsu Kai, Hiromi Ikeura, Ariaki Ayanokoji, Masahiko Tamaki
KEYWORDS:
Phytoremediation, Zinnia, 5-Aminolevulinic Acid, Soil Total Petroleum Hydrocarbon (TPH) Concentration, Soil Dehydrogenase Activity (DHA)
JOURNAL NAME:
Journal of Agricultural Chemistry and Environment,
Vol.9 No.3,
August
11,
2020
ABSTRACT: In this
study, we compared plant height, weight, soil TPH concentration, and soil DHA
level after 18 weeks of Zennia hybrida cultivation with four different concentrations of 5-Aminolevulinic acid
(ALA)-based liquid fertilizer: 1500-fold, 5000-fold, and 8000-fold dilutions,
along with a non-treatment control of diluted ALA. The plants of ALA-treated
were significantly taller than the non-treatment control. The plants of
ALA-treated plants were higher in shoot fresh weight, shoot dry weight, and
root dry weight than the non-treatment control. The plot of ALA-based liquid
fertilizer with the 5000-fold dilution was significantly highest in shoot fresh
weight, shoot dry weight, and root dry weight. ALA-treated plants were lower in
the soil Total Petroleum Hydrocarbon (TPH) concentration than the non-treatment control. The plot of ALA-based
liquid fertilizer with the 5000-fold was significantly lowest in the TPH
concentration. In addition, ALA-treated plants were higher in the soil dehydrogenase activity (DHA) than the non-treatment
control. The plot of ALA-based liquid fertilizer with the 5000-fold was significantly
highest in the TPH concentration. This study indicated that ALA-applied
zinnia-grown oil-contaminated soil is more effective than not. The remediation
in oil-contaminated soil with ALA-based liquid fertilizer is more effective
than the non-treatment control; furthermore, ALA application with 5000-fold dilution was most suitable in
oil-contaminated soil among other plots.