TITLE:
Transcriptome Analysis of White-Rot Fungi in Response to Lignocellulose or Lignocellulose-Derived Material Using RNA Sequencing Technology
AUTHORS:
Yixing Zhang, Koichi Yamaura
KEYWORDS:
Transcriptome, RNA-Seq, White-Rot Fungi, Lignocellulosic Biomass
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.11 No.8,
August
6,
2020
ABSTRACT: White-rot fungi are the only organisms that can
completely degrade all components of lignocellulosic biomass, including the
recalcitrant lignin polymer. Lignin degradation is important for the industrial
application of lignocellulosic biomass as a raw material for producing
value-added chemicals and materials.
Therefore, elucidating the lignin degradation mechanism in white-rot fungi will help researchers develop efficient and eco-friendly methods enabling
the production of value-added chemicals from lignocellulosic biomass. A
transcriptome analysis is an effective way to compare gene expression patterns
between different samples under diverse conditions and can provide insights
into biological processes. The democratization of next-generation sequencing
technology, especially RNA-sequencing, has made transcriptome sequencing and
analysis a common research approach for many laboratories. In this review, we focus on the transcriptome
profiles of two well-characterized white-rot fungi (Phanerochaete chrysosporium and Dichomitus squalens) in response to various
lignocellulosic materials. The application of RNA-seq technology combining with
other techniques remains the best approach for investigating fungal secretomes
and elucidating the mechanisms of fungal responses to lignocellulose.