TITLE:
The Amplification and Application of Ribosomal RNA (rDNA) Gene Sequences of Blidingia minima (Chlorophyta, Blidingia)
AUTHORS:
Yuan Ao, Weijie Shen, Anxiang Yuan, Yi Yin, Yuan He, Songdong Shen
KEYWORDS:
Blidingia minima, rDNA, IGS (Intergenic Spacer), Intraspecies Relationships
JOURNAL NAME:
Open Journal of Marine Science,
Vol.10 No.3,
July
23,
2020
ABSTRACT: The sequence of the ribosomal RNA gene (rDNA) plays
an important role in species identification and phylogenetic analysis. However,
the only published full-length sequence of a
ribosomal gene of green algae is that of Ulva mutabilis. In this
study, we amplified the full-length sequence of each
ribosomal gene unit of the ribosomal gene of Blidingia minima. The
full-length sequence of the ribosomal gene in Blidingia minima is 8676
bp, including the 1759 bp 18S rDNA, 576 bp internal transcribed spacer (ITS) + 5.8S
rDNA, 3282 bp 28S rDNA, and 3059 bp
intergenic spacer (IGS) region. We then carried out a series of genetic
analyses based on the ITS and IGS sequences, to verify whether IGS sequences are useful for studying the genetic
diversity of green algae from different locations. We amplified the IGS
sequences of Blidingia minima from 10 different locations in the Yellow
Sea. Multiple alignments of the IGS sequences of samples from these 10 different sites revealed varying degrees of base differences, and comparative analysis
of the ITS sequences revealed that our amplified species was classified as Blidingia
minima and distinct from other green algae. In conclusion, our full-length
amplified ribosomal gene provides useful information to enrich the data on
green algae ribosomal genes and provides an effective molecular marker
for the analysis of the interspecies and intraspecies relationships of Blidingia minima.