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C. Zdero, F. Bohlmann, A. Anderberg and R. M. King, “Eremophilane Derivatives and Other Constituents from Haeckeria Species and Further Australian Inuleae,” Phytochemistry, Vol. 30, No. 8, 1991, pp. 2643-2650. doi:10.1016/0031-9422(91)85114-F

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• TITLE: Efficient Isolation of an Anti-Cancer Sesquiterpene Lactone from Calomeria amaranthoides by Steam Distillation

  AUTHORS: Colin Charles Duke, Caroline van Haaften, Van Hoan Tran

  KEYWORDS: Sesquiterpene Lactone, Calomeria amaranthoides, Steam Distillation, Chromatography

  JOURNAL NAME: Green and Sustainable Chemistry, Vol.1 No.4, November 16, 2011

  ABSTRACT: An efficient method of isolating an anti-cancer sesquiterpene lactone, eremophila-1(10)-11(13)-dien-12,8?- olide, was developed from fresh Calomeria amaranthoides plant material on the basis of its non-polar vola-tile property. Steam distillation of fresh plant material gave a high recovery of sesquiterpene-rich oil, 0.56%, compared with the estimated 0.66% calculated from solvent extraction of dried plant material. The ses-quiterpene-rich oil containing 58% of the sesquiterpene lactone was fractionated by the short-column vac-uum chromatography method, using minimal stationary-phase and solvent, to give the sesquiterpene lactone 95% purity, yield 41%.