TITLE:
Comparative Paraquat Sensitivity of Newly Germinated and Mature Fronds of the Aquatic Macrophyte Spirodela polyrhiza
AUTHORS:
Jihae Park, Hojun Lee, Taejun Han
KEYWORDS:
Spirodela polyrhiza, Paraquat, Herbicide, Chlorophyll Contents, Chlorophyll a Fluorescence, Root Length
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.11 No.7,
July
13,
2020
ABSTRACT: Here, we compared the intrinsic characteristics of 3-day-(newly
germinated; “young”) and 8-week-old (“mature”)
fronds of the aquatic plant Spirodela polyrhiza and their sensitivity to paraquat, a toxic
herbicide. Endpoints measured were frond area and fresh weight, root length, chlorophyll a and b contents, and chlorophyll a fluorescence. Significant differences were detected in the intrinsic physiological
traits between young and mature fronds. Young fronds showed higher root length,
chlorophyll contents, maximum quantum yield (Fv/Fm),
maximal relative electron transport rate (rETRmax) and saturating photon
flux density (PFD), whereas mature fronds exhibited greater frond area and fresh
weight. After a 72 h exposure to paraquat, root length and rETRmax were
identified as the most sensitive endpoints of paraquat toxicity for both frond types,
with EC50 values of 0.66 and 0.76 μg·L-1 for young fronds,
respectively, and 5.53 and 2.28 μg·L-1 for mature fronds, respectively.
Young fronds of S. polyrhiza showed significantly higher sensitivity to paraquat than mature fronds. A survey
of other studies on paraquat toxicity to Lemna species revealed that EC50 values of paraquat-induced inhibition of root regrowth and rETRmax in
both stages were the lowest, indicating that these two endpoints were the most sensitive
to paraquat. In addition, EC50 values of both endpoints of mature fronds
of S. polyrhiza appear
to be similar to the current allowable concentrations in drinking water set by the
World Health Organization (WHO), indicating that these values may have application
for the assessment of toxicity risk of paraquat in aquatic ecosystems.