TITLE:
Bacteriostatic Effects of Hyaluronan-Based Bioresorbable Membrane
AUTHORS:
Keiichi Uchida, Kohei Otake, Mikihiro Inoue, Yuhki Koike, Kohei Matsushita, Koji Tanaka, Yasuhiro Inoue, Yasuhiko Mohri, Masato Kusunoki
KEYWORDS:
HA/CMC, Bacteriostatic, Hyaluronan, Adhesion, Surgery
JOURNAL NAME:
Surgical Science,
Vol.2 No.9,
November
14,
2011
ABSTRACT: Purpose: The purpose of this study is to determine the presence of bacteriostatic effects of hyaluronan-based bioresorbable membrane (HA/CMC) on selected major bacterial strains in digestive organs. Methods: We firstly evaluated the growth inhibition effect of HA/CMC for E. coli and S. aureus by determining the optical density (OD)650 in the incubation medium. At second, to determine the viable counts of bacteria, total adenosine triphosphate (ATP) were measured with five groups; several concentrations of HA/CMC and control. Results: OD curve gradually elevated and reached to plateau at 4 hours in E. coli. and 6 hours in S. aureus. After reaching plateau, the growth inhibition of both strains was statistically significantly correlated to the concentrations of HA/CMC. The ATP productions had statistically significant differences at 6 hours after incubation and inhibited in dose-dependence of a well-dissolved HA/CMC. Conclusion: HA/CMC may have dose-dependently bacteriostatic effects on S. aureus and E. coli.