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Pan, W.Y., Ngo, T.T.M., Camunas-Soler, J., Song, C.X., Kowarsky, M., Blumenfeld, Y.J., et al. (2017) Simultaneously Monitoring Immune Response and Microbial Infections during Pregnancy through Plasma cfRNA Sequencing. Clinical Chemistry, 63, 1695-1704. https://doi.org/10.1373/clinchem.2017.273888

has been cited by the following article:

• TITLE: ExRNA Sequencing Results of Different Volumes of Serum

  AUTHORS: Liujing Wang, Xiangwei Zhao

  KEYWORDS: Extracellular RNAs, Serum, RNA-seq Technology, Serum Volume

  JOURNAL NAME: Journal of Biosciences and Medicines, Vol.8 No.6, June 30, 2020

  ABSTRACT: Circulating extracellular RNAs (exRNAs) are very important potential biomarkers of disease. But few have been reported the extracellular RNA profiles in serum. In this study, we tried to use RNA-seq technology to analyze the extracellular RNA by using low volume peripheral blood serum and compared the sequencing results between different volumes of serum (500 μl, 1.5 ml, 2.5 ml, 3.5 ml). According to the results, we found that most of the exRNA in the serum is lncRNA, protein_coding and MT-rRNA. And when the volume of serum is between 0.5 - 3.5 ml, the numbers and reads of exRNA, including the reads of circRNA, have not changed significantly. It indicates that the sequencing result of exRNA in serum has no significance with the initial serum volume.