TITLE:
Antioxidant, Anti-Inflammatory Efficacy and HPLC Analysis of Annona muricata Leaves Extracts from Republic of Benin
AUTHORS:
Kohonou Arnaud, Chabi Nicodème, Dah-Nouvlessounon Durand, Nounagnon Martial, Sognigbe Basile, Sina Haziz, N’Tcha Christine, Kohonou A. Christian, Lehmane Halfane, Dougnon Victorien, Pacôme Noumavo, Baba-Moussa Lamine
KEYWORDS:
A. muricata, Secondary Metabolites Extracts, Screening, Biological Activities, Benin
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.11 No.6,
June
24,
2020
ABSTRACT: Annona muricata L. (Soursop or
Graviola) is a naturally occurring plant seen in Southern part of Africa,
traditionally used in Benin to treat various diseases. The present study aimed
to investigate phytochemical composition and antioxidant, anti-inflammatory
activities of A. muricata leaves extracts. The secondary metabolites of ethanolic
and hemi-ethanolic extracts were analysed by HPLC method. The DPPH and FRAP
methods were used to evaluate the antioxidant activity. Inhibition of albumin
denaturation method was used to evaluate anti-inflammatory activity of the
tested extracts of which larval cytotoxicity was studied. The major identified compounds were gallic acid, chlorogenic acid,
cafeic acid, tannic acid, ferrulic acid, Rutin. Ascorbic acid exhibited the
highest inhibition percentage (83.33% ± 0.50%) of DPPH radical with the lowest
IC50 (45.1 ± 0.28 μg/ml). The inhibition of the ferric ion Fe3+ varied (p = 0.0013) according to the
extracts type. IC50 values of ferric ion inhibition range from 119.5
± 3.10 to 250.8 ± 2.13 μg/ml respectively for A. muricata leaves
ethanol and hemi-ethanolic extracts. The hemi-ethanolic extract exhibited the
highest anti-inflammatory activity (96.66% ± 1.17%). The presence of
phenolic compound confers to A. muricata leaves, through the ethanolic
and the hemi-ethanolic extracts, the antioxidant and anti-inflammatory
activities.