TITLE:
Phytochemical Characterization of Three Plants and Their Antisickling Activity in the Management of Sickle Cell Disease
AUTHORS:
Tatiana Kangah Mireille Kplé, Joel Akakpo-Akue, Julien Koffi Golly, Yvette Fofie, Marcel Gnamien Ahon, Mattieu Adou Kra, Ibrahime Sanogo, Antoinette Chiayé C. Yapo-Crezoit
KEYWORDS:
Sickle Cell Disease, Antisickling Activity, Hydro-Ethanolic Extract, J. gossypiifolia, P. nigrescens, J. secunda, Côte d’Ivoire
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.8 No.6,
June
5,
2020
ABSTRACT: Natural plant products have been used by the
population of the south-eastern part of Côte d’Ivoire in the management of
sickle cell anemia. This study was aimed at investigating the antisickling
activity of the hydro-ethanolic extract of a combination of the
leaves of J. secunda, J. gossypiifolia and P. nigrescens. These three plants species were used in the Ivorian
traditional herbal medicine. Preliminary phytochemistry was carried out using
standard methods. As for the sickling reversal test, the Hb SS blood sickling
was induced with 2% sodium metabisulfite. After 120 minutes of incubation, the
plant extract was added. Every 30 minutes for a period of 120 minutes, a drop
of the prepared solution was observed at (40×) magnification and the
percentage of reversion calculated. The phytochemical analysis revealed the
presence of Alkaloids, Flavonoids, Polyphenols, Catechic Tannins, Sterols and
Polyterpenes. The results of the reversal test showed that the percentage of
sickling reversal effect of the combination of the plants (75.00 ± 4.33b,c)
was highly superior than the negative control (10.17 ± 0.55d) but
was significantly (p J. secunda: 83.50 ± 2.33a; J. gossypiifolia: 78.00 ± 3.67b,c and P. nigrescens:
77.83 ± 2.89b,c) and the positive control (80.66 ± 2.22a,b).
From the results, the extracts J. secunda, J. gossypiifolia and P. nigrescens have shown to be
therapeutically beneficial to the population. Their use is also justified in
the management of sickle cell disease in the south-eastern part of Côte
d’Ivoire.