TITLE:
Hepatoprotective Effects of the Leaves of Agauria salicifolia against Acetaminophen-Induced Liver Injury in Mice
AUTHORS:
Mathias K. Tsague, Lionel C. K. Bomgning, Christian K. Fofié, Elvine P. Nguelefack-Mbuyo, Agathe L. Fotio, Télesphore Benoît Nguelefack
KEYWORDS:
Agauria salicifolia, Hepatoprotection, Antioxidant, Histology
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.8 No.6,
June
3,
2020
ABSTRACT: Agauria salicifolia (Ericaceae) is a medicinal
plant traditionally used for the treatment of liver ailments. The present study
investigates the hepatoprotective effect of the residual aqueous fraction (RAF)
of Agauria salicifolia on acetaminophen (APAP)-induced liver damage. The
ethanol extract obtained as maceration of the dried leaves, was fractionated into
hexane, ethyl acetate and residual aqueous fractions. Adult mice of both sexes
were pre-treated with the reference drug silymarin (50 mg/kg) or RAF (100 and
200 mg/kg) during 6 days followed by a single administration of APAP (500
mg/kg) on day 7. The hepatoprotective effect and the contribution of
antioxidant activities were evaluated by determining the level of transaminases
in serum samples, the levels of proteins, nitric oxide (NO), malondialdehyde
(MDA), glutathione (GSH), superoxide dismutase (SOD), and catalase in liver
homogenates. Histological analyses of liver slides were also performed. RAF at
the doses of 100 and 200 mg/kg and silymarin significantly (p increase in proteins
(p p p p