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Pitt, J.A., Kozal, J.S., Jayasundara, N., Massarsky, A., Trevisan, R., Geitner, N., Wiesnerd, M., Levine, E.D. and Di Giulio, R.T. (2018) Uptake, Tissue Distribution, and Toxicity of Polystyrene Nanoparticles in Developing Zebrafish (Danio rerio). Aquatic Toxicology, 194, 185-194.
https://doi.org/10.1016/j.aquatox.2017.11.017

has been cited by the following article:

• TITLE: Production of Soybean Oil Nanoemulsion (SONE) and Evaluation of Angiogenic and Embryotoxic Activity

  AUTHORS: Tamíris Augusto Marinho, Patrícia Silva Nunes, Paulo Roberto de Melo-Reis, Mônica Rodrigues Ferreira Machado, Nelson Jorge Silva Júnior

  KEYWORDS: Soybean Oil, Angiogenesis Inducing Agents, Toxicity, Biotechnology, Nanotechnology

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.11 No.3, June 2, 2020

  ABSTRACT: The SONE oil/water was prepared using the pseudo-ternary phase diagram, using a low energy method with phase inversion by changing the composition. In order to optimize the preparation of SONE, three speeds were used in the agitator arm and two different rods. The particle size and polydispersity index were determined by Dynamic Light Scattering (DLS) and the stability test by the freeze-thaw cycle. Angiogenesis in chicken embryo egg chorioallantoic membrane and zebrafish (Danio rerio) embryotoxicity was performed. The lower stirring speed and propeller shaft induced smaller particle size (550.2 nm). Regarding angiogenesis, there was a statistically significant difference for all the evaluated parameters (length, caliber, junctions, and number of blood vessel complexes) and the result was higher in SONE when compared to the inhibition control (Dexamethasone), but lower than the induction control (Regederm®) and there was no statistically significant difference between SONE and distilled water. It was observed that the exposure of the zebrafish embryos to SONE caused an increase in the mortality rate dependent on time and concentration. The LC50 for SONE decreased statistically with increasing exposure (p-value = 0.046). Heart rate decreased significantly with increasing concentration at all exposure times (p-value