TITLE:
Structural Characterization of Ulvan Polysaccharide from Cultivated and Collected Ulva fasciata (Chlorophyta)
AUTHORS:
Tiphane Andrade Figueira, Antonio Jorge Ribeiro da Silva, Alex Enrich-Prast, Yocie Yoneshigue-Valentin, Vinícius Peruzzi de Oliveira
KEYWORDS:
Sulfated Polysaccharide, Aquaculture, FTIR Analysis, NMR Analysis, Biotechnology
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.11 No.5,
May
22,
2020
ABSTRACT: Ulvan is a sulfated heteropolysaccharide present in
the cell wall of Ulva species with
unique structural properties and technological potential. Here we characterized
by FTIR and NMR analysis the structure of ulvan from Ulva fasciata collected in natural environment (SEA) and after in vitro biomass cultivation in
nutrient enriched water (CULT). FTIR spectrum of CULT ulvan presented stronger
signals of sulfate groups than SEA. 1H and 13C NMR showed
that both ulvan are composed mainly of ulvanobiuronic acid 3-sulfate type A and
type B. SEA ulvan presented signals characteristics of xylose, suggesting the
presence of ulvanobiose in its structure, while CULT presented most signals of
type A disaccharide. The cultivation of Ulva could be an alternative to suffice the emerging demand for ulvan meeting requirements
of quality and quantity.