TITLE:
Optimization of Solvent Systems for the Extraction of Vitexin as the Major Bioactive Flavonoid in Prosopis farcta
AUTHORS:
Somaieh Zafari, Mohsen Sharifi
KEYWORDS:
Prosopis farcta, Vitexin, Solvent System, HPLC Technique
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.11 No.5,
May
7,
2020
ABSTRACT: Prosopis farcta, a plant belongs to the mimosoideae,
is characterized by a very wide spectrum of various bioactive and medical
constituents. Vitexin, the marker flavonoid found in Prosopis, has
potent and broad antitumour efficacy in
preclinical models. Many studies had been done for the isolation of flavonoids
(vitexin) by completely different chromatographically methodology. During this study, vitexin was isolated from Prosopis farcta by 6 different extraction methods in which parameters as the type,
concentration and pH of the
extracting solvents considered. Among different solvent systems used,
methanol-water (40%, containing acetic acid 0.5%) was found to be the best
solvent generating the highest yield (0.554 mg·g-1 DW) from Prosopis leaves. The present work suggests an efficient method for estimation the
greatest content of vitexin analyzed by HPLC technique and introduces Prosopis farcta as a suitable source of this
flavonoid with several pharmacological properties.