TITLE:
Advance of Multiplex PCR in Rapid Detecting Transgenic Soybean Oil
AUTHORS:
Yi Liu, Siya Yin
KEYWORDS:
Transgenic Soybean Oil, DNA Extraction Method, Multiplex PCR
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.11 No.3,
March
24,
2020
ABSTRACT: Transgenic food safety is a high-profile public
health issue in worldwide, especially transgenic soybean (Glycine max L.) oil. To rapidly and effectively detect transgenic components of soybean oil, in the
present study, we isolated DNA from transgenic soybean oil by modified method,
and employed the multiplex PCR method to identify targeted genes, including
CaMV35S promoter, Nos terminator, NPTII, CP4-EPSPS and endogenous gene Lectin. The research aims to build a
method which is accurate, rapid and reliable for detection of genetically
modified soybeans oil. The targeted gene including DNA was successfully
established by the improved method, and then amplified by PCR. Five genes are
simultaneously specifically detected. Commercial soybean, genetically modified
soy bean and oil were detected with the
Multiplex PCR. The improved method of DNA extraction was rapid and accurate to
extract high quality total DNA which was amplified by PCR. The method could
eliminate the PCR inhibitor. A way of detecting the genetically modified
soybean and Oil was set up in this study.