TITLE:
Identification of AFLP Markers Linked to Leaf Rust Resistance Genes Using Near Isogenic Lines of Wheat
AUTHORS:
Navjot Kaur Dhillon, Harcharan Singh Dhaliwal
KEYWORDS:
AFLP, Leaf Rust, Wheat, Lr9, Isogenic Lines
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.2 No.5,
November
25,
2011
ABSTRACT: The present investigation was undertaken to find molecular markers linked to leaf rust resistance genes, Lr9 and Kharchia local mutant KLM4-3B. Preliminary AFLP analysis was carried out with different stocks, a survey of primer combinations with different selective nucleotide indicated that for each primer combination, the number of scorable loci ranged from 34 to 123. Only a limited primer combination used in the set of parental and near isogenic lines showed a high level of polymorphism for AFLP marker. Putative AFLP marker were found to be linked to Lr9, Lr19 and KLM4-3B. The alien genes were readily identified.