TITLE:
Evaluation the Possibility of Using of miRNA-21 as a Prognostic and Predictive Factor in Colorectal Cancer (South Egypt Experience)
AUTHORS:
Ashraf Zedain, Hosny Badrawy, Mayada Fawzy, Dina Ismail Abd El Razik, Sanaa Saber, Abeer Ibrahim
KEYWORDS:
CRC, miRNA-21, Prognostic, Predictive
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.11 No.2,
February
28,
2020
ABSTRACT: Background: Micro RNA 21(miRNA-21) is overexpressed in numerous solid types of tumors, indicating that its biology is relevant to numerous types of cancer. However,in Colorectal Cancer (CRC)there is a lot of studiesconfirmed its use as diagnostic marker, other studies suggested it could be used as a predictive marker for 5 Fu sensitivity so this study was conducted to verified the significance of using miRNA-21 as a prognostic and predictive factor in CRC. Patients and Methods: It is a prospective study that included 45 patients with stage I-IV CRC who attended at South Egypt Cancer Institute from April 2017 to October 2018.miRNA-21 was measured by (q PCR) from peripheral blood.Response to 5-FU containing regimens was assessed using RECISTcriteria and its levels of expression were correlated to the clincopathological data,response and survival of the patients.Results:High expression of the miRNA21 was significantly associated with advanced stage of CRC p 0.001,Lymph Node (LN)invasion p 0.001,distant metastasis p0.01 lymphovascular (LVI) invasion p 0.01 and pre-treatment level of CarcinoEmbryonic Antigen (CEA) p 0.031. Also we found that high expression level significantly badly affected the response to 5-Fu containing regimen p 0.001, DFS p 0.01.Conclusion:miRNA-21 could be used as non-invasive prognostic and predictive marker in CRC.