TITLE:
Investigation of Ovarian Follicular Waves and Major Hormonal Profile in Red Chittagong Cattle
AUTHORS:
Saiful Islam, Md. Ahsanul Kabir, Md. Faizul Hossain Miraz, Effat Jahan Tamanna, Satya Ranjan Sarker, Zinia Islam, S. M. Jahangir Hossain, Sharif Hossain, Gautam Kumar Deb
KEYWORDS:
Red Chittagong Cattle, Ovarian Follicles, Progesterone, Follicle Stimulating Hormone, Luteinizing Hormone, ELISA
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.11 No.2,
February
18,
2020
ABSTRACT: Red Chittagong Cattle (RCC) is one of the renowned
varieties of cattle which is indigenous and most potential type of domestic
animal genetic resource in Bangladesh with distinct phenotypic characteristics.
Reproductive management might be a process for multiplying this cattle variety.
Therefore, the goals of this study were to characterize the follicular waves of
RCC and to determine the Progesterone (P4), Follicle Stimulating
Hormone (FSH) and Luteinizing Hormone (LH) profile during three consecutive
estrus cycle. Follicular wave emergence (Day 0) was identified using
trans-vaginal ultrasound technique and follicular growth was then monitored
from Day 0 to Day 23 during three consecutive estrus cycle and blood samples
were collected. After measuring the growth and diameter of the follicles,
hormonal assays of collected blood samples were performed using Enzyme Linked
Immunosorbent Assay (ELISA). We found that, both RCC cows and heifers showed
two waves inter-ovulatory intervals. The length of inter-ovulatory intervals
and the diameter of the ovulatory follicle of RCC cows and heifers were
slightly different from each other. The identification of the dominant follicle
was confirmed from the start of its deviation from other subordinate follicles
till it reached to the maximum diameter and identified as dominant follicle
which increases linearly for approximately six days (growing phase). This
dominant follicle is remained approximately same size for five days (static
phase), and then begins to regress (regressing phase) decreasing the antral
diameter. The follicular waves are first detectable as 4 - 5 mm follicles
approximately at day 0 and day 10 both for RCC cows and heifers which showed
two waves inter-ovulatory intervals. The circulating varied concentrations of
LH, FSH and P4 were determined using ELISA in case of both cows and
heifers and the concentration curves followed the similar pattern of change
during the estrus cycle. Hence, the knowledge about ovarian follicular waves
will create a base line for reproductive management of RCC as well as to allow
reproductive biotechnologies to improve and multiply this cattle variety.