TITLE:
Composition and Molecular Weight Distribution of Albumin and Globulin Protein Isolates from Durum Wheat Genotypes
AUTHORS:
Daniel Hailegiorgis, Fikru Mekonnen, Faris Hailu, Chong Ae Lee, Song Joong Yun
KEYWORDS:
Albumin, Durum Wheat, Globulin, Polymorphism, Seed Protein
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.11 No.2,
February
17,
2020
ABSTRACT: This paper attempts to evaluate the banding patterns
of non-gluten protein isolates from the grain of durum wheat varieties. Under
reduced condition, polyacrylamide-gel electrophoresis has revealed a number of
different sized albumin and globulin protein bands. The electrophoretic pattern
of globulin showed more polymorphisms than that of albumin. High polymorphism,
both in band intensity and occurrence, was observed between 15 kDa and 35 kDa.
Most of the protein bands were scored in the range of 10 kDa and 85 kDa in the
two protein fractions. At a cutoff point 2.5, cluster analysis based on the
SDS-PAGE of globulin proteins classified the durum wheat varieties into three
major family groups. Generally, the experiment showed the suitability and
usefulness of globulin protein fractions as a genetic marker in discriminating
durum wheat genotypes.